A1287
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A1287
Sigma
5-Azacytidine
Hybri-Max™, lyophilized powder, γ-irradiated, hybridoma tested
| Synonym: | Ladakamycin, 4-Amino-1-(β- |
| CAS Number: | 320-67-2 |
| Linear Formula: | C8H12N4O5 |
| Molecular Weight: | 244.20 |
| Beilstein Registry Number: | 620461 |
| EC Number: | 206-280-2 |
| MDL number: | MFCD00006539 |
| PubChem Substance ID: | 24890525 |
Description
| Frequently Asked Questions | Live Chat and Frequently Asked Questions are available for this Product. |
| Reconstitution | Reconstitute contents of vial with 10 mL sterile cell culture medium. Stock solution is sufficient to prepare 500ml medium. Final working concentration: 10 μM 5-azacytidine. |
| Biochem/physiol Actions | A potent growth inhibitor and cytotoxic agent; inhibits DNA methyltransferase, an important regulatory mechanism of gene expression, gene activation and silencing. |
| Legal Information | Hybri-Max is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| grade | Hybri-Max™ |
| sterility | γ-irradiated |
| form | lyophilized powder |
| total impurities | Endotoxin, tested |
| mp | 226-232 °C (dec.)(lit.) |
| suitability | hybridoma tested |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type P2 (EN 143) respirator cartridges |
| Hazard Codes | T |
| Risk Statements | 45-46-22 |
| Safety Statements | 53-22-36/37/39-45 |
| WGK Germany | 3 |
| RTECS | XZ3017500 |
References
| reference | Kusaba H., et al., Association of 5′-CpG demethylation and altered chromatin structure in the promoter region with transcriptional activation of the multidrug resistance 1 gene in human cancer cells. Eur. J. Biochem. 262, 924-932, (1999) |
| Broday L., et al., 5-Azacytidine induces transgene silencing by DNA methylation in Chinese hamster cells. Mol. Cell. Biol. 19, 3198-3204, (1999) | |
| Qian X., et al., DNA methylation regulates p27kip1 expression in rodent pituitary cell lines. Am. J. Pathol. 153, 1475-1482, (1998) | |
| Canova C., et al., Epigenetic control of programmed cell death: inhibition by 5-azacytidine of 1,25-dihydroxyvitamin D3-induced programmed cell death in C6.9 glioma cells. Mech. Ageing Dev. 101, 153-166, (1998) | |
| Merck | Merck 13,893 |
| Aldrich MSDS 1, 139:B / Corp MSDS 1 (1), 321:D / FT-IR 2 (3), 3896:C / FT-IR 1 (2), 831:D / FT-NMR 1 (3), 418:B / IR-Spectra (2), 1191:F / IR-Spectra (3), 1367:E / RegBook 1 (2), 2603:A / Sax 6, 332 / Sigma FT-IR 1 (1), 758:C / Structure Index 1, 411:B:5 |
